rat kidney epithelial cell line nrk 52e  (Procell Inc)

Journal: Antioxidants (Basel, Switzerland)

Article Title: Inhibition of Indoxyl Sulfate-Induced Reactive Oxygen Species-Related Ferroptosis Alleviates Renal Cell Injury In Vitro and Chronic Kidney Disease Progression In Vivo.

doi: 10.3390/antiox12111931

Figure Lengend Snippet: Figure 2. IS induced ER stress, ferroptosis, and epithelial–mesenchymal transition (EMT) through ROS signaling in the renal tubular cells. NRK-52E cells were treated with various concentrations of IS (0.1–1 mM) for 24 h with or without pretreatment of 1 mM NAC for 1 h or 50 µM deferoxamine (DFO) for 1 h. (A,B) Western blot analysis and quantification for ER stress-related GRP78, p-eIF2α, ATF4, ATF6, and CHOP (A), and ferroptosis-related GPX4, PTGS2, and xCT (B) protein expression. (C) Western blot analysis and quantification for ATF4, CHOP, GPX4, and PTGS2 protein expression in IS-treated NRK-52E cells with or without NAC. (D) Western blot analysis and quantification for EMT-related fibronectin, E-cadherin, and vimentin protein expression in the IS-treated NRK-52E cells with or without NAC or DFO. Data are presented as means ± SD (n = 6 in (A–D); independent experiments). *, p < 0.05, compared with the control (C) group. #, p < 0.05, compared with the corresponding concentrations of IS groups. One-way ANOVA followed by Tukey’s post hoc test was used to analyze statistical differences.

Article Snippet: A normal rat kidney epithelial cell line (NRK-52E) obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA) was used.

Techniques: Western Blot, Expressing, Control